Human Cleaved Caspase-7 (Asp198) ELISA Kit (ab275900)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21 pg/ml
- Range: 281.25 pg/ml - 18000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cleaved Caspase-7 (Asp198) ELISA Kit
See all Caspase-7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Jurkat 8 4% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
21 pg/ml -
Range
281.25 pg/ml - 18000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 105 97% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cleaved Caspase-7 (Asp198) ELISA kit (ab275900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Caspase-7 protein in human cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cleaved Caspase-7 (Asp198) with 21 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Caspase-7 is a cytoplasmic cysteine protease involved in the activation cascade of caspases responsible for execution of apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs). Caspase-7 cleaves poly(ADP-ribose) polymerase (PARP). Caspase-7 overexpression promotes programmed cell death. Caspase-7 is expressed as an inactive pro-form (pro caspase-7). In apoptosis, the pro caspase-7 is activated by proteolytic cleavages at Asp23-Ala24, Asp198-Ser199 and Asp206-Ala207 bonds catalyzed by granzyme B and caspase-10, generating two subunits p20 and p11 that are assembled into hetero-tetrameric active enzyme. This kit measures Caspase-7 only upon cleavage of Asp198-Ser199 bond targeting the p20 subunit.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cleaved Caspase-7 (Asp198) Capture Antibody 1 x 600µl 10X Human Cleaved Caspase-7 (Asp198) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Cleaved Caspase-7 (Asp198) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Apoptotic protease Mch-3
- Apoptotic protease MCH3
- CASP-7
see all -
Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cleaved Caspase-7 (Asp198) standard curve in 1X Cell Extraction Buffer PTR.The Cleaved Caspase-7 (Asp198) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cleaved Caspase-7 (Asp198)The concentrations of Cleaved Caspase-7 (Asp198) were measured in duplicate and interpolated from the Cleaved Caspase-7 (Asp198) standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cleaved Caspase-7 (Asp198) concentration was determined to be 10,244 pg/mL in etoposide treated Jurkat extract, 9,741 pg/mL in staurosporine treated Jurkat extract and 4,367 pg/mL in staurosporine treated HeLa extract samples.
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Comparison of Jurkat cell extracts treated with 50 µM etoposide for 4 hours vs mock treated, Jurkat and HeLa cell extracts treated with 1 µM staurosporine for 4 hours vs mock treated.The concentrations of Cleaved Caspase-7 (Asp198) were measured in duplicate and interpolated from the Cleaved Caspase-7 (Asp198) standard curve. The interpolated values are plotted in pg of Cleaved Caspase-7 (Asp198) per µg of extract (mean +/- SD, n=2). The mean concentration was determined to be 19 pg/µg in Jurkat (etoposide treated), 3 pg/µg in Jurkat (etoposide mock treated), 33 pg/µg in Jurkat (staurosporine treated), undetectable in Jurkat (staurosporine mock treated), 9 pg/µg in HeLa (staurosporine treated) and undetectable in HeLa (staurosporine mock treated). Note that cell treatments with apoptosis inducers, etoposide and staurosporine, known to induce Caspase-7 cleavage, greatly increase Cleaved Caspase-7 cellular concentration when measured with this kit.
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Sandwich ELISA - Human Cleaved Caspase-7 Elisa Kit (Asp198) (ab275900)To learn more about the advantages of recombinant antibodies see here.
