Anti-IL-9 antibody - C-terminal (ab181397)
Key features and details
- Rabbit polyclonal to IL-9 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-9 antibody - C-terminal
See all IL-9 primary antibodies -
Description
Rabbit polyclonal to IL-9 - C-terminal -
Host species
Rabbit -
Specificity
ab181397 is predicted not to cross-react with IL7. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-9 (C terminal). (16 amino acid peptide; NP_000581).
Database link: P15248 -
Positive control
- WB: Human spleen tissue and spleen tissue lysate; Hek 293 and HeLa lysate. IHC: Human spleen tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-9 antibody - C-terminal (ab181397)Lane 1 : Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml
Lane 2 : Anti-IL-9 antibody - C-terminal (ab181397) at 2 µg/ml
Lane 3 : Anti-IL-9 antibody - C-terminal (ab181397) at 4 µg/ml
All lanes : Human IL-9 recombinant protein, 20 ng
Secondary
All lanes : Goat anti Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 15 kDa
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Western blot - Anti-IL-9 antibody - C-terminal (ab181397)All lanes : Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml
Lane 1 : Human spleen lysate
Lane 2 : Hek 293 lysate
Lane 3 : HeLa cell spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG HRP conjugate
Predicted band size: 15 kDa
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Western blot - Anti-IL-9 antibody - Conformation-Specific (ab181397)Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml + Human spleen tissue lysate at 15 µg
Predicted band size: 15 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody - Conformation-Specific (ab181397)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling IL9 with ab181397 at 20 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody - Conformation-Specific (ab181397)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling IL9 with ab181397 at 5 µg/ml.
