Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SOX11 antibody [EPR20457] (ab229185)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20457] to SOX11
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-SOX11 antibody [EPR20457]
See all SOX11 primary antibodies
Description

Rabbit monoclonal [EPR20457] to SOX11
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SH-SY5Y whole cell lysate; Human fetal brain lysate. IHC-P: Human mantle cell lymphoma and glioma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20457
Isotype

IgG
Research areas

Western blot - Anti-SOX11 antibody [EPR20457] (ab229185)

All lanes : Anti-SOX11 antibody [EPR20457] (ab229185) at 1/1000 dilution

Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 2 : Human fetal brain lysate at 10 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 47 kDa

Exposure time : Lane 1: 1 minute, Lane 2: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is phosphorylated form, consistent with what has been described in the literature. (PMID:19880779, PMID:29973868).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX11 antibody [EPR20457] (ab229185)

Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining in human mantle cell lymphoma (PMID: 19880788. PMID:17934069) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX11 antibody [EPR20457] (ab229185)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining on human glioma (PMID:15583815.) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-SOX11 antibody [EPR20457] (ab229185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com