Anti-SOX11 antibody (ab134739)
Key features and details
- Rabbit polyclonal to SOX11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOX11 antibody
See all SOX11 primary antibodies -
Description
Rabbit polyclonal to SOX11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SOX11 aa 250-350 conjugated to keyhole limpet haemocyanin.
Database link: P35716 -
Positive control
- This antibody gave a positive signal in Human Brain Adult and Fetal tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RabbitHorsePigChimpanzeeMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).Target
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Function
Probably important in the developing nervous system. May also have a role in tissue modeling during development. -
Tissue specificity
Expressed mainly in the nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6664 Human
- Omim: 600898 Human
- SwissProt: P35716 Human
- Unigene: 432638 Human
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Alternative names
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
see all
Images
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Western blot - Anti-SOX11 antibody (ab134739)All lanes : Anti-SOX11 antibody (ab134739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa (possible non-specific binding)
Exposure time: 150 secondsThe predicted molecular weight of SOX11 is 46kDa (SwissProt), however we expect to observe a banding pattern around 70kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134739 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab134739 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SOX11 antibody (ab134739)All lanes : Anti-SOX11 antibody (ab134739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa (possible non-specific binding)
Exposure time: 150 secondsThe predicted molecular weight of SOX11 is 46kDa (SwissProt), however we expect to observe a banding pattern around 70kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134739 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
