Anti-LEF1 antibody (ab74549)
Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LEF1 antibody
See all LEF1 primary antibodies -
Description
Rabbit polyclonal to LEF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human LEF1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab95919) -
Positive control
- Purchase matching WB positive control:Recombinant Human LEF1 protein
- This antibody gave a positive signal in Jurkat whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitChickenPigMonkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 42, 50 kDa (predicted molecular weight: 44 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42, 50 kDa (predicted molecular weight: 44 kDa).Target
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Function
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. -
Tissue specificity
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. -
Sequence similarities
Belongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
Domain
Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. -
Cellular localization
Nucleus. Found in nuclear bodies upon PIASG binding. - Information by UniProt
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Database links
- Entrez Gene: 51176 Human
- Entrez Gene: 16842 Mouse
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: P27782 Mouse
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
see all -
Alternative names
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
Images
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Western blot - Anti-LEF1 antibody (ab74549)Anti-LEF1 antibody (ab74549) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab74549 detects a band at 42 and 50 kDa. While this differs to its predicted molecular weight of 44 kDa, this migration has been observed in the literature PMID:17335794.
References (0)
ab74549 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-LEF1 antibody (ab74549)Anti-LEF1 antibody (ab74549) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab74549 detects a band at 42 and 50 kDa. While this differs to its predicted molecular weight of 44 kDa, this migration has been observed in the literature PMID:17335794.
