Human Securin ELISA Kit (ab230935)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 64 pg/ml
- Range: 102 pg/ml - 6500 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Securin ELISA Kit
See all Securin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Securin 5 6.4% Inter-assay Sample n Mean SD CV% Securin 3 5.2% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
64 pg/ml -
Range
102 pg/ml - 6500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 98 89% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Securin ELISA Kit (ab230935) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Securin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Securin with 64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Securin Capture Antibody 1 x 600µl 10X Human Securin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human Securin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. -
Tissue specificity
Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer. -
Sequence similarities
Belongs to the securin family. -
Developmental stage
Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded. -
Domain
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue. -
Post-translational
modificationsPhosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Alternative names
- AW555095
- C87862
- Cut2
see all -
Database links
- Entrez Gene: 9232 Human
- Omim: 604147 Human
- SwissProt: Q6IAL9 Human
- SwissProt: O95997 Human
- Unigene: 350966 Human
Images
-
Other - Human Securin ELISA Kit (ab230935)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human Securin standard curve in 1X Cell Extraction Buffer PTR.The Securin standard curve was prepared as described in Section 10.
-
Interpolated concentrations of native Securin in Human Daudi, U937, and HUVEC Cell Extracts based on a 62.5 µg/mL, 250 µg/mL, and 250 µg/mL extract loads respectively.The concentrations of Securin were measured in duplicate and interpolated from the Securin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Securin concentration was determined to be 4123 pg/mL in Daudi Cell Extract, 2086 pg/ml in U937 Cell Extract, and 2017 pg/mL in HUVEC Cell Extract.
-
Sandwich ELISA - Human Securin ELISA Kit (ab230935)To learn more about the advantages of recombinant antibodies see here.
