Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)
![Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)](https://www.abcam.com/ps/products/251/ab251190/Images/ab251190-410324-anti-grsf1-antibody-epr16679-bsa-and-azide-free-western-blot.jpg "Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16679] to GRSF1 - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-GRSF1 antibody [EPR16679] - BSA and Azide free
See all GRSF1 primary antibodies -
Description
Rabbit monoclonal [EPR16679] to GRSF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251190 is the carrier-free version of ab194358. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251190 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16679 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)All lanes : Anti-GRSF1 antibody [EPR16679] (ab194358) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDaThis data was developed using ab194358, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)](https://www.abcam.com/ps/products/251/ab251190/Images/ab251190-3-anti-grsf1-antibody-epr16679-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)This data was developed using ab194358, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)](https://www.abcam.com/ps/products/251/ab251190/Images/ab251190-2-anti-grsf1-antibody-epr16679-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)This data was developed using ab194358, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GRSF1 with ab194358 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue). -
![Flow Cytometry - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)](https://www.abcam.com/ps/products/251/ab251190/Images/ab251190-1-anti-grsf1-antibody-epr16679-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)This data was developed using ab194358, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling GRSF1 (red) with purified ab194358 at dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody. -
![Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)](https://www.abcam.com/ps/products/251/ab251190/Images/ab251190-5-benefits-of-recombinant-antibodies.png)
Anti-GRSF1 antibody [EPR16679] - BSA and Azide free (ab251190)
