Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4883] to KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883]

  • Description

    Rabbit monoclonal [EPR4883] to KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181)

  • Host species

    Rabbit

  • Specificity

    ab124841 recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TAOK1. The exact sequence is proprietary. Synthetic peptide corresponding to residues surrounding Serine 181 of Human TAO kinase 2 (Q9UL54) or Serine 181 of TAO kinase 1 (Q7L7X3) or Serine 177 of TAO kinase 3 (Q9H2K8).

  • Positive control

    • 293T cell lysates and Human colonic adenocarcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)
    Western blot - Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)
    All lanes : Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841) at 1/1000 dilution

    Lane 1 : Untreated 293T cell lysates
    Lane 2 : Lambda Phosphatase treated 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 138 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)

    ab124841, at 1/250 to 1/500, staining TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) in Human colonic adenocarcinoma by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)
    Anti-KDS (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841)

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