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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Abi-1 antibody (ab65828)

Anti-Abi-1 antibody (ab65828)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Abi-1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Abi-1 antibody
    See all Abi-1 primary antibodies
  • Description

    Rabbit polyclonal to Abi-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Abi-1 aa 483-494 (C terminal).
    Sequence:

    WYEGVCNRVTGL


    Database link: Q8IZP0
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.025% Sodium azide
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)

    Abi 1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65828 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)

    Abi 1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65828 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex  with DAB as the chromogen.

  • Western blot - Anti-Abi-1 antibody (ab65828)
    Western blot - Anti-Abi-1 antibody (ab65828)
    All lanes : Anti-Abi-1 antibody (ab65828) at 0.5 µg/ml

    Lane 1 : rat brain tissue lysates
    Lane 2 : mouse brain tissue lysates
    Lane 3 : human THP-1 whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab65828)

    Immunohistochemical analysis of rat brain tissue labeling Abi-1 with ab65828 at 0.5 μg/mL. 

  • Western blot - Anti-Abi-1 antibody (ab65828)
    Western blot - Anti-Abi-1 antibody (ab65828)
    Anti-Abi-1 antibody (ab65828) at 2 µg/ml + Rat brain lysate

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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