Anti-Abi-1 antibody (ab263960)
Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Abi-1 antibody
See all Abi-1 primary antibodies -
Description
Rabbit polyclonal to Abi-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Mouse Abi-1 (C terminal).
Database link: Q8CBW3 -
Positive control
- WB: Abi-1 recombinant protein; Mouse and rat brain lysates;. HCT-116, HeLa and NIH/3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Abi-1 antibody (ab263960)All lanes : Anti-Abi-1 antibody (ab263960) at 1/1000 dilution (overnight at 4degC)
Lane 1 : MEF3 KO lysate
Lane 2 : MEF3 WT lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : HCT-116 whole cell lysate
Lane 6 : HeLa whole cell lysate
Lane 7 : NIH/3T3 whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Peroxidase rabbit secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 55 kDa
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Western blot - Anti-Abi-1 antibody (ab263960)Anti-Abi-1 antibody (ab263960) at 1/1000 dilution (overnight at 4degC) + Abi-1 recombinant protein, 50 ng
Secondary
Peroxidase rabbit secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 55 kDa
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Western blot - Anti-Abi-1 antibody (ab263960)All lanes : Anti-Abi-1 antibody (ab263960) at 1/1000 dilution (2 hrs at RT)
Lane 1 : Purified recombinant His-tagged Abi1 Isoform 2
Lane 2 : Purified recombinant His-tagged Abi1 Isoform 3
Lysates/proteins at 2 µl per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit for 60 min at RT at 1/10000 dilution
Predicted band size: 55 kDaBlocking buffer: 5% non-fat milk in TBS-t overnight at 4°C.
