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Anti-GRK1 antibody [D11] (ab2776)

Key features and details

  • Mouse monoclonal [D11] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GRK1 antibody [D11]
    See all GRK1 primary antibodies

  • Description

    Mouse monoclonal [D11] to GRK1

  • Host species

    Mouse

  • Specificity

    Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Cow, Human

  • Immunogen

    Full length native protein (purified) corresponding to Human GRK1.

  • Epitope

    This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunolocalization of GRK1 in bovine retina using ab2776.

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