Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [G8] to GRK1
Suitable for: ICC/IF
Reacts with: Mouse, Cow, Human
Isotype: IgG1

Product name

Anti-GRK1 antibody [G8]
See all GRK1 primary antibodies
Description

Mouse monoclonal [G8] to GRK1
Host species

Mouse
Specificity

Detects G-protein-associated rhodopsin kinase 1a (GRK1a).
Tested applications

Suitable for: ICC/IFmore details
Species reactivity

Reacts with: Mouse, Cow, Human
Immunogen

Full length native protein (purified) corresponding to Human GRK1.
Epitope

This antibody recognizes an epitope in the C-terminal region of GRK1a.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Concentration information loading...
Purity

Protein G purified
Primary antibody notes

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.
Clonality

Monoclonal
Clone number

G8
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Immunolocalization of GRK1 in bovine retina using ab2775.

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