Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Wednesday March 10, 2021

Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15376(B)] to GKN2
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-GKN2 antibody [EPR15376(B)]
See all GKN2 primary antibodies
Description

Rabbit monoclonal [EPR15376(B)] to GKN2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human stomach tissue and lysates.
General notes
This product was previously labelled as Gastrokine 2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15376(B)
Isotype

IgG
Research areas

Western blot - Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Anti-GKN2 antibody [EPR15376(B)] (ab184163) at 1/50000 dilution + Human stomach lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

The observed MW is lower than the predicted 20kDa, which may be due to the removing of a 20 amino acid signal peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab184163 at 1/500 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Immunoprecipitation of Human stomach lysates (lane 1) or PBS (lane 2) using ab184163. Detection of GKN2 utilised ab184163 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.
Anti-GKN2 antibody [EPR15376(B)] (ab184163)

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