Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15376(B)] to GKN2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GKN2 antibody [EPR15376(B)]
    See all GKN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15376(B)] to GKN2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human stomach tissue and lysates.

  • General notes

     

     

     This product was previously labelled as Gastrokine 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15376(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GKN2 antibody [EPR15376(B)] (ab184163)
    Western blot - Anti-GKN2 antibody [EPR15376(B)] (ab184163)
    Anti-GKN2 antibody [EPR15376(B)] (ab184163) at 1/50000 dilution + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The observed MW is lower than the predicted 20kDa, which may be due to the removing of a 20 amino acid signal peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15376(B)] (ab184163)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15376(B)] (ab184163)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab184163 at 1/500 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-GKN2 antibody [EPR15376(B)] (ab184163)
    Immunoprecipitation - Anti-GKN2 antibody [EPR15376(B)] (ab184163)

    Immunoprecipitation of Human stomach lysates (lane 1) or PBS (lane 2) using ab184163. Detection of GKN2 utilised ab184163 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.

     

     

  • Anti-GKN2 antibody [EPR15376(B)] (ab184163)
    Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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