Anti-Cathepsin H antibody [AF14D7] (ab88960)
![Anti-Cathepsin H antibody [AF14D7] (ab88960)](https://www.abcam.com/ps/products/88/ab88960/Images/ab88960-412567-anti-cathepsin-h-antibody-af14d7-western-blot-human-knockout-lysate.jpg "Anti-Cathepsin H antibody [AF14D7] (ab88960)")
Key features and details
- Mouse monoclonal [AF14D7] to Cathepsin H
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cathepsin H antibody [AF14D7]
See all Cathepsin H primary antibodies -
Description
Mouse monoclonal [AF14D7] to Cathepsin H -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant human Cathepsin H with His tag purified from E.coli
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Positive control
- WB: HEK-293T, K562, HepG2, HT29 and C6 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, HEPES -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
AF14D7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Cathepsin H antibody [AF14D7] (ab88960)All lanes : Anti-Cathepsin H antibody [AF14D7] (ab88960) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CTSH knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab88960 observed at 27 kDa. Red - loading control ab181602 observed at 36 kDa.
ab88960 Anti-Cathepsin H antibody [AF14D7] was shown to specifically react with Cathepsin H in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266245 (knockout cell lysate ab258387) was used. Wild-type and Cathepsin H knockout samples were subjected to SDS-PAGE. ab88960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cathepsin H antibody [AF14D7] (ab88960)](https://www.abcam.com/ps/products/88/ab88960/Images/ab88960-104478-anti-cathepsin-h-antibody-af14d7-western-blot.jpg)
Western blot - Anti-Cathepsin H antibody [AF14D7] (ab88960)All lanes : Anti-Cathepsin H antibody [AF14D7] (ab88960) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : C6 cell lysate
Predicted band size: 37 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
