Anti-Sprouty 4/Spry-4 antibody (ab228712)
Key features and details
- Rabbit polyclonal to Sprouty 4/Spry-4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit polyclonal to Sprouty 4/Spry-4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow
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Immunogen
Recombinant fragment within Human Sprouty 4/Spry-4 (internal sequence). The exact sequence is proprietary.
Database link: Q9C004 -
Positive control
- WB: Mouse brain lysate; NT2/D1 whole cell lysate. IHC-P: U87 xenograft tissue; Mouse duodenum tissue.
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General notes
Previously labelled as Sprouty 4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sprouty 4/Spry-4 antibody (ab228712)Anti-Sprouty 4/Spry-4 antibody (ab228712) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 33 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab228712)Paraffin-embedded U87 xenograft tissue stained for Sprouty 4/Spry-4 using ab228712 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Sprouty 4/Spry-4 antibody (ab228712)Anti-Sprouty 4/Spry-4 antibody (ab228712) at 1/500 dilution + NT2/D1 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 33 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab228712)Paraffin-embedded mouse duodenum tissue stained for Sprouty 4/Spry-4 using ab228712 at 1/500 dilution in immunohistochemical analysis.
