Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)
![Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)](https://www.abcam.com/ps/products/238/ab238934/Images/ab238934-3-ab176337203607IP1.jpg "Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, MCF7, HeLa, and K562 cell lysates. IP: MCF7 cell lysate.
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General notes
ab238934 is the carrier-free version of ab176337 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238934 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Sprouty 4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS
r8 -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12127 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)
Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176337).
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![Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)](https://www.abcam.com/ps/products/238/ab238934/Images/ab238934-4-benefits-of-recombinant-antibodies.png)
Anti-Sprouty 4/Spry-4 antibody [EPR12127] - BSA and Azide free (ab238934)
