Anti-Sprouty 4/Spry-4 antibody (ab7513)
Key features and details
- Rabbit polyclonal to Sprouty 4/Spry-4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit polyclonal to Sprouty 4/Spry-4This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Sprouty 4/Spry-4 aa 283-299 conjugated to keyhole limpet haemocyanin.
Sequence:CKAASGDAKTSRPDKPF
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General notes
Previously labelled as Sprouty 4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)
Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD. -
Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)All lanes : Anti-Sprouty 4/Spry-4 antibody (ab7513) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : SW13 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDaThe blot was incubated with ab7516 (1/100) overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)ab7513 at 2.5 µg/ml staining human heart tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)ab7513 staining tumour cells in human liver tissue. Brown color indicates presence of protein, blue color shows cell nuclei.
