Anti-Crk p38 antibody [EP242Y] (ab45136)
![Anti-Crk p38 antibody [EP242Y] (ab45136)](https://www.abcam.com/ps/products/45/ab45136/Images/ab45136-393544-anti-crk-p38-antibody-ep242y-western-blot-human-knockout-lysate.jpg "Anti-Crk p38 antibody [EP242Y] (ab45136)")
Key features and details
- Rabbit monoclonal [EP242Y] to Crk p38
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Crk p38 antibody [EP242Y]
See all Crk p38 primary antibodies -
Description
Rabbit monoclonal [EP242Y] to Crk p38 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human Crk p38 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and HepG2 cell lysates. ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP242Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136)All lanes : Anti-Crk p38 antibody [EP242Y] (ab45136) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CRK knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab45136 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.
ab45136 Anti-Crk p38 antibody [EP242Y] was shown to specifically react with Crk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265097 (knockout cell lysate ab257899) was used. Wild-type and Crk knockout samples were subjected to SDS-PAGE. ab45136 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136)](https://www.abcam.com/ps/products/45/ab45136/Images/ab45136-57285-anti-crk-p38-antibody-ep242y-western-blot.jpg)
Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136)Anti-Crk p38 antibody [EP242Y] (ab45136) at 1/10000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-Crk p38 antibody [EP242Y] (ab45136)](https://www.abcam.com/ps/products/45/ab45136/Images/ab45136-57284-anti-crk-p38-antibody-ep242y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Crk p38 antibody [EP242Y] (ab45136)Immunofluorescent staining of HeLa cells using ab45136 at a 1/250 dilution.
