Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Crk p38 antibody [EPR4984(2)] (ab133581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4984(2)] to Crk p38
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Crk p38 antibody [EPR4984(2)]
    See all Crk p38 primary antibodies

  • Description

    Rabbit monoclonal [EPR4984(2)] to Crk p38

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 79%  identity (78% positives) with CRKL. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human Crk p38 (UniProt ID: P46108)

  • Positive control

    • WB: HepG2, K562, HeLa, and Jurkat cell lysates Flow cyt: HepG2 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4984(2)

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
    Flow Cytometry - Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
    ab133581, at a 1/100, staining Crk p38 in permeabilized HepG2 cells using flow cytometry analysis (Red). Green curve corresponds to a control Rabbit IgG antibody (negative control).
  • Western blot - Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
    Western blot - Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
    All lanes : Anti-Crk p38 antibody [EPR4984(2)] (ab133581) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
    Anti-Crk p38 antibody [EPR4984(2)] (ab133581)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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