Recombinant human TAK1 protein (ab132950)
Key features and details
- Expression system: Wheat germ
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB, ELISA
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Product name
Recombinant human TAK1 protein
See all TAK1 proteins and peptides -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVV CKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPV CLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPK ALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEV FEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPP LIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQY PCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLES KLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIA ATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTR SHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHC KMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDE NKSLSTYYQQCKKQLEVIRSQQQKRQGTS -
Predicted molecular weight
89 kDa including tags -
Amino acids
1 to 579
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human TAK1 protein (ab132950)12.5% SDS-PAGE analysis of ab132950 stained with Coomassie Blue.
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Functional Studies - Recombinant human TAK1 protein (ab132950)Kinase assay using ab132950 and [gamma 32P] ATP.
Fig 1: SDS-PAGE gel
Fig 2: Autoradiograph of SDS-PAGE gelKinase substrates:
H3 histone, family 3A - calf thymus - Molecular weight: approx. 16.5-17 kDa on SDS-PAGE
Myelin basic protein - bovine brain - Molecular weight: approx. 21-22 kDa on SDS-PAGE
Histone H1.0 - calf thymus - Molecular weight: approx. 32-33 kDa on SDS-PAGE
Beta-casein protein - bovine milk - Molecular weight: approx. 33-34 kDa on SDS-PAGE