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Signal Transduction Protein Trafficking ER Proteins

Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)

Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12294] to RAB11-FIP2 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free
    See all RAB11-FIP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12294] to RAB11-FIP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RAB11-FIP2. The exact sequence is proprietary.
    Database link: Q7L804

  • General notes

    ab249812 is the carrier-free version of ab174313 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249812 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12294
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Images

  • Western blot - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    Western blot - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    All lanes : Anti-RAB11-FIP2 antibody [EPR12294] (ab174313) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 58 kDa



    This data was developed using ab174313, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    This data was developed using ab174313, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using ab174313 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Immunoprecipitation - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    Immunoprecipitation - Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    This data was developed using ab174313, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from 293T cell lysate (lane 1) or 1XPBS (negative control) (lane 2) using ab174313 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)
    Anti-RAB11-FIP2 antibody [EPR12294] - BSA and Azide free (ab249812)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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