Anti-Caveolin-1 antibody [SP43] - C-terminal (ab227636)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP43] to Caveolin-1 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Caveolin-1 antibody [SP43] - C-terminal
See all Caveolin-1 primary antibodies -
Description
Rabbit monoclonal [SP43] to Caveolin-1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human lung carcinoma, placenta, liver hepatocellular carcinoma, lung, skeletal muscle, liver, cerebellum, cerebral cortex, colon and HK lymphoma tissues
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP43 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human lung, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227636 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227636 for 30 mins at room temperature. -
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human placenta tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human lung tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human colon tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.
-