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Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y]
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)
  • Host species

    Rabbit
  • Specificity

    ab75876 detects Caveolin-1 phosphorylated at tyrosine 14.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A673, Insulin treated A673, A673 treated with insulin then the membrane was incubated with phosphatase, HeLa and HeLa cell treated with pervanadate whole cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2288Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/1000 dilution

    Lane 1 : Untreated A673 (Human muscle Ewing's Sarcoma) whole cell lysates
    Lane 2 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates
    Lane 3 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa


    Exposure time: 30 seconds


    Blocking/Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

    Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
    Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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