Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y]
See all Caveolin-1 primary antibodies -
Description
Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14) -
Host species
Rabbit -
Specificity
ab75876 detects Caveolin-1 phosphorylated at tyrosine 14. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A673, Insulin treated A673, A673 treated with insulin then the membrane was incubated with phosphatase, HeLa and HeLa cell treated with pervanadate whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2288Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/1000 dilution
Lane 1 : Untreated A673 (Human muscle Ewing's Sarcoma) whole cell lysates
Lane 2 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates
Lane 3 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Exposure time: 30 secondsBlocking/Diluting buffer 5% NFDM/TBST
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All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
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Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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