Anti-Caveolin-1 antibody (ab36152)
Key features and details
- Goat polyclonal to Caveolin-1
- Suitable for: Flow Cyt (Intra), ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caveolin-1 antibody
See all Caveolin-1 primary antibodies -
Description
Goat polyclonal to Caveolin-1 -
Host species
Goat -
Specificity
No cross-reactivity expected to Caveolin-2 and Caveolin-3. -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
Sequence:DELSEKQVYDAH
Database link: Q03135 -
Positive control
- ICC: U-2 OS and A431 cells; WB: A549 and A431 cell lysates. Human heart tissue lysate. Flow Cyt (Intra): HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml + Human Heart lysate at 35 µg
Predicted band size: 20 kDa
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Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 µg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Lane 1 : Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml
Lane 2 : Anti-Caveolin-1 antibody (ab36152) at 0.001 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa
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Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
