FirePlex Human Inflammation - Immunoassay Panel (ab243550)
Overview
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Product name
FirePlex Human Inflammation - Immunoassay Panel -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Multiplex -
Assay time
3h 30m -
Species reactivity
Reacts with: Human -
Product overview
FirePlex Human Inflammation - Immunoassay Panel (ab243350) contains all the reagents you need to run a 8-plex assay: antibody conjugated particles, Human Core Reagents and Protein Standard Mix.
NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.
Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.
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Notes
This panel was previously available under the product code ab231391/ab229793, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.
The following information provides the sensitivity, range, and required Protein Standard Mix.
Target
Sensitivity
(pg/mL)Dynamic Range
(pg/mL)Protein
StandardIFN gamma
2.25
4.57 - 10,000
Mix A
IL-1 beta
0.47
1.52 - 3,333
Mix A
IL-6
0.15
0.51 - 1,111
Mix A
IL-8
0.29
0.51 - 1,111
Mix A
IL-10
1.12
4.57 - 10,000
Mix A
IL-12p70
0.64
4.57 - 10,000
Mix A
MCP1
0.28
1.52 - 3,333
Mix A
TNF alpha
5.36
13.7 - 10,000
Mix A
Negative Control Particles
N/A
N/A
N/A -
Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Human Inflammation Capture Particles (ab229793) 1 x 1.65ml 10X Wash Buffer 1 x 25ml 15X Human Inflammation Biotin Detectors (ab229793) 1 x 367µl 2X Human & NHP Assay Diluent 1 x 15ml 5X Reporter Solution 1 x 4ml 96-well microplate with a filter 1 unit Filter Plate Seal 1 x 3 units Human Multiplex Immunoassay Protein Standard Mix A 1 vial Tungsten cleaning wire 1 unit -
Research areas
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Cellular localization
IL-12: Secreted. TNF alpha: Secreted and Cell membrane. IL-8: Secreted. MCP1: Secreted. IL-6: Secreted. IL-10: Secreted. Interferon gamma: Secreted. IL-1 beta: Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. -
Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 3553 Human
- Entrez Gene: 3569 Human
- Entrez Gene: 3576 Human
- Entrez Gene: 3586 Human
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Entrez Gene: 6347 Human
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Images
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Assay Summary
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Example of Human Inflammation (8-plex) standard curvesMean background-subtracted data values are graphed (+/- SD).
