Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24708-25] to c-Kit - BSA and Azide free
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Mouse

Overview

  • Product name

    Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free
    See all c-Kit primary antibodies

  • Description

    Rabbit monoclonal [EPR24708-25] to c-Kit - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICCmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: P815 cells. Flow Cyt: Mouse bone marrow cells.

  • General notes

    ab281843 is the carrier-free version of ab273119. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281843 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24708-25

  • Isotype

    IgG

Images

  • Immunocytochemistry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)
    Immunocytochemistry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)

    This data was developed using ab273119, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of  0.1% TritonX-100 permeabilized P815 cells labelling c-Kit with ab273119 at 1/50 (10.28 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in P815 cell line. Negative control: NIH/3T3 (PMID: 10523834) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)
    Flow Cytometry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)

    This data was developed using ab273119, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-Lineage Cocktail (CD3+Gr-1+CD11b+CD45R+Ter-119) conjugated to FITC. Gated on viable cells.

  • Flow Cytometry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)
    Flow Cytometry - Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)

    This data was developed using ab273119, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD34 conjugated to BV421. Gated on viable cells.

  • Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)
    Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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