Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)
![Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)](https://www.abcam.com/ps/products/249/ab249599/Images/ab249599-365883-anti-tom1-antibody-epr11989b-western-blot-knockout-hap1-tom1-hepg2-hela-human-lysates.jpg "Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11989(B)] to TOM1 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free
See all TOM1 primary antibodies -
Description
Rabbit monoclonal [EPR11989(B)] to TOM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab249599 is the carrier-free version of ab170928. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249599 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11989(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TOM1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170928).
Lanes 1 - 4: Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)](https://www.abcam.com/ps/products/249/ab249599/Images/ab249599-2-benefits-of-recombinant-antibodies.png)
Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)
