Human RHOA knockout HEK-293T cell lysate (ab257637)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260309 - Human RHOA knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human RHOA knockout HEK293T cell lysate (ab257637)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: RHOA knockout HEK-293T cell lysate (20µg)
Lane 3: Wild-type HEK-293T cell lysate (20µg)
Lane 4: RHOA knockout HEK-293T cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab187027 observed at 21 kDa. Red - loading control ab7291 observed at 50 kDa.
ab187027 Anti-RhoA antibody [EPR18134] was shown to specifically react with RhoA in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266592 (knockout cell lysate ab257637) was used. Wild-type and RhoA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187027 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 5000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human RHOA knockout HEK293T cell lysate (ab257637)Allele-1: 4 bp insertion in exon 2
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Sanger Sequencing - Human RHOA knockout HEK293T cell lysate (ab257637)Allele-2: Insertion of the selection cassette in exon 2
