Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NCS1 antibody [EPR7699] (ab129166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7699] to NCS1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NCS1 antibody [EPR7699]
    See all NCS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7699] to NCS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NCS1 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • U-87 MG, SH-SY5Y, A549, 293T and Human fetal brain lysates; Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7699

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NCS1 antibody [EPR7699] (ab129166)
    Western blot - Anti-NCS1 antibody [EPR7699] (ab129166)
    All lanes : Anti-NCS1 antibody [EPR7699] (ab129166) at 1/1000 dilution

    Lane 1 : U-87 MG cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : A549 cell lysates
    Lane 4 : 293T cell lysates
    Lane 5 : Fetal brain tissue lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • Flow Cytometry - Anti-NCS1 antibody [EPR7699] (ab129166)
    Flow Cytometry - Anti-NCS1 antibody [EPR7699] (ab129166)
    Overlay histogram showing SH-SY5Y cells stained with ab129166 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129166, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCS1 antibody [EPR7699] (ab129166)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCS1 antibody [EPR7699] (ab129166)

    ab129165, at 1/250 dilution staining NCS1 in paraffin-embedded Human brain tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-NCS1 antibody [EPR7699] (ab129166)
    Anti-NCS1 antibody [EPR7699] (ab129166)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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