Human Calbindin Matched Antibody Pair Kit (ab220139)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 1.95 pg/ml - 125 pg/ml
Overview
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Product name
Human Calbindin Matched Antibody Pair Kit
See all Calbindin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.3 pg/ml -
Range
1.95 pg/ml - 125 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Calbindin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Calbindin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab218270. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Calbindin Capture Antibody 2 x 50µg 1 x 50µg Human Calbindin Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Calbindin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
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Alternative names
- avian-type
- CAB27
- CALB
see all -
Database links
- Entrez Gene: 793 Human
- Omim: 114050 Human
- SwissProt: P05937 Human
- Unigene: 65425 Human
Images
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Human Calbindin standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Calbindin Matched Antibody Pair Kit (ab220139)To learn more about the advantages of recombinant antibodies see here.
