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Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins

Human Calbindin Antibody Pair - BSA and Azide free (ab241917)

Human Calbindin Antibody Pair - BSA and Azide free (ab241917)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Calbindin Antibody Pair - BSA and Azide free
    See all Calbindin kits
  • Assay type

    ELISA set
  • Range

    1.95 pg/ml - 125 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Calbindin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab218270), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Calbindin Capture Antibody (unconjugated) 1 x 100µg
    Human Calbindin Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
  • Function

    Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
  • Sequence similarities

    Belongs to the calbindin family.
    Contains 5 EF-hand domains.
  • Domain

    This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
  • Target information above from: UniProt accession P05937 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • avian-type
    • CAB27
    • CALB
    • CALB 1
    • CALB1
    • CALB1_HUMAN
    • Calbindin
    • Calbindin 1 28kDa
    • Calbindin D28
    • D 28K
    • D-28K
    • D28K
    • OTTHUMP00000166027
    • OTTHUMP00000225441
    • RTVL H protein
    • Vitamin D dependent calcium binding protein
    • Vitamin D dependent calcium binding protein avian type
    • Vitamin D-dependent calcium-binding protein
    see all
  • Database links

    • Entrez Gene: 793 Human
    • Omim: 114050 Human
    • SwissProt: P05937 Human
    • Unigene: 65425 Human

    Images

    • Sandwich ELISA - Human Calbindin Antibody Pair - BSA and Azide free (ab241917)
      Sandwich ELISA - Human Calbindin Antibody Pair - BSA and Azide free (ab241917)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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      Human Calbindin Matched Antibody Pair Kit (ab220139)

      Applications:

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