Calretinin ELISA Kit (ab233616)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.2 pg/ml
- Range: 234 pg/ml - 15000 pg/ml
- Sample type: Milk, Mouse brain extract, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Calretinin ELISA Kit
See all Calretinin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Brain 6 4.5% Inter-assay Sample n Mean SD CV% Brain 3 10.5% -
Sample type
Milk, Tissue Extracts, Mouse brain extract -
Assay type
Sandwich (quantitative) -
Sensitivity
4.2 pg/ml -
Range
234 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 111 103% - 115% Tissue Extracts 107 98% - 114% Mouse brain extract 106 97% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Calretinin ELISA Kit (ab233616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calretinin protein in milk, mouse brain extract, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Calretinin with 4.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Calretinin Capture Antibody 1 x 600µl 10X Calretinin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Calretinin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the calbindin family.
Contains 6 EF-hand domains. - Information by UniProt
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Alternative names
- 29 kDa calbindin
- CAB 29
- CAB29
see all -
Database links
- Entrez Gene: 794 Human
- Entrez Gene: 12308 Mouse
- Entrez Gene: 117059 Rat
- Omim: 114051 Human
- SwissProt: P22676 Human
- SwissProt: Q08331 Mouse
- SwissProt: P47728 Rat
- Unigene: 106857 Human
see all
Images
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Other - Calretinin ELISA Kit (ab233616)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Calretinin standard curve in 1X Cell Extraction Buffer PTR.The Calretinin standard curve was prepared as described in Section 10.
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Interpolated concentrations of native Calretinin in human, mouse, and rat brain extract samples.The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Calretinin in human breast milk samples.The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calretinin concentration was determined to be 227 ng/mL in neat human breast milk.
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Relative expression of calretinin in normal tissue extractsCalretinin expression in non-neural and non-malignant tissues are expected to be significantly lower than neural tissues. Interpolated concentrations of native Calretinin in human brain, heart, liver, and placenta extract samples. The concentrations of Calretinin were measured in duplicates, interpolated from the Calretinin standard curves and corrected for sample dilution. Undiluted samples are as follows: brain extract 3 µg/mL, heart, liver, and placenta extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Calretinin ELISA Kit (ab233616)To learn more about the advantages of recombinant antibodies see here.
