Anti-CD1b antibody [EP7251] (ab173576)
![Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-197078-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg "Anti-CD1b antibody [EP7251] (ab173576)")
Key features and details
- Rabbit monoclonal [EP7251] to CD1b
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CD1b antibody [EP7251]
See all CD1b primary antibodies -
Description
Rabbit monoclonal [EP7251] to CD1b -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CD1b aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P29016 -
Positive control
- Fetal thymus and Jurkat lysates, Human spleen tissue, Jurkat cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7251 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1b with ab173576 at 1/100 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-221509-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)ab173576 showing +ve staining in Human normal thymus tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-221508-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)ab173576 showing +ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-201851-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)ab173576 showing -ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-201850-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)ab173576 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-201849-anti-cd1b-antibody-ep7251-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (ab173576)ab173576 showing -ve staining in Human endometrial adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-197079-anti-cd1b-antibody-ep7251-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] (ab173576)Immunofluorescent staining of Jurkat cells labeling CD1b with ab173576 at 1/50 dilution
-
![Western blot - Anti-CD1b antibody [EP7251] (ab173576)](https://www.abcam.com/ps/products/173/ab173576/Images/ab173576-197075-anti-cd1b-antibody-ep7251-western-blot.jpg)
Western blot - Anti-CD1b antibody [EP7251] (ab173576)All lanes : Anti-CD1b antibody [EP7251] (ab173576) at 1/1000 dilution
Lane 1 : Human fetal thymus lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
