Human IL-2 Receptor ELISA Kit (ab46036)
Key features and details
- Sensitivity: 32.5 pg/ml
- Range: 68.75 pg/ml - 2220 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-2 Receptor ELISA Kit
See all IL-2 Receptor alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% 1 6 1594pg/ml 184 11.6% 2 6 1528pg/ml 135 8.8% 3 6 1705pg/ml 204 12% 4 6 1124pg/ml 171 15.2% 5 6 2305pg/ml 165 7.2% 6 6 1824pg/ml 126 69% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32.5 pg/ml -
Range
68.75 pg/ml - 2220 pg/ml -
Recovery
87 %
Sample specific recovery Sample type Average % Range Serum 99 86% - 122% -
Assay time
3h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-2 Receptor Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 Receptor in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2 Receptor.
A monoclonal antibody specific for IL-2 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 Receptor present in the samples.
This kit will recognize both endogenous and recombinant Human IL-2 Receptor.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-IL-2 Receptor Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-2 Receptor Control Silver 2 vials 4 vials IL-2 Receptor Microplate (12 x 8 well strips) 1 unit 2 units Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 1 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Interleukin 2 receptor alpha chain
- CD25
- CD25 antigen
see all -
Database links
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab46036