Anti-RPL32 antibody (ab229758)
Key features and details
- Rabbit polyclonal to RPL32
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL32 antibody -
Description
Rabbit polyclonal to RPL32 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human RPL32 aa 1 to the C-terminus.
Database link: P62910 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human lung cancer and colon cancer tissue. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPL32 antibody (ab229758)All lanes : Anti-RPL32 antibody (ab229758) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa15% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (ab229758)Paraffin-embedded human lung cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL32 antibody (ab229758)Paraffin-embedded human colon cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-RPL32 antibody (ab229758)4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPL32 (green) using ab229758 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).