Human CD86 ELISA Kit (ab242239)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.5 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human CD86 ELISA Kit
See all CD86 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% protein 8 2% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
3.5 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD86 ELISA Kit (ab242239) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD86 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD86 with 3.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD86 Capture Antibody 1 x 600µl 10X Human CD86 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CD86 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- Activation B7-2 antigen
- Activation B7-2 antigen 3
- B-lymphocyte activation antigen B7-2
see all -
Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
Images
-
Other - Human CD86 ELISA Kit (ab242239)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human CD86 standard curve in Sample Diluent NS.The CD86 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native CD86 in human HDLM-2 cell culture supernatant samples.The concentrations of CD86 were measured in duplicates, interpolated from the CD86 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 7,134 pg/mL in HDLM-2 supernatant.
-
Sandwich ELISA - Human CD86 ELISA Kit (ab242239)To learn more about the advantages of recombinant antibodies see here.
