Anti-HspBP1 antibody (ab3858)
Key features and details
- Rabbit polyclonal to HspBP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HspBP1 antibody -
Description
Rabbit polyclonal to HspBP1 -
Host species
Rabbit -
Specificity
This antibody recognises HspBP1 (39kDa) in Western Blots. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Fusion protein corresponding to Human HspBP1.
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Positive control
- This antibody gave a positive signal in HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 4 ARM repeats. - Information by UniProt
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Database links
- Entrez Gene: 23640 Human
- Omim: 612939 Human
- SwissProt: Q9NZL4 Human
- Unigene: 53066 Human
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Alternative names
- 1500019G21Rik antibody
- FES1 antibody
- Heat shock protein 70 binding protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)
ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
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Western blot - Anti-HspBP1 antibody (ab3858)Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutesWestern blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.
Protocols
Datasheets and documents
References (0)
ab3858 has not yet been referenced specifically in any publications.
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)
ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
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Western blot - Anti-HspBP1 antibody (ab3858)Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutesWestern blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.