Anti-Pbx3 antibody (ab129478)
Key features and details
- Rabbit polyclonal to Pbx3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Pbx3 antibody
See all Pbx3 primary antibodies -
Description
Rabbit polyclonal to Pbx3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Pbx3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: O35317 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: E14 Mouse Embryonic Brain; E16 Mouse Embryonic Brain; P0 Mouse Brain; P7 Mouse Brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatHorseChickenHumanChimpanzeeMacaque monkeyChinese hamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.Notes WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.Target
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Function
Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 417093 Chicken
- Entrez Gene: 5090 Human
- Entrez Gene: 18516 Mouse
- Entrez Gene: 311876 Rat
- Omim: 176312 Human
- SwissProt: P40426 Human
- SwissProt: O35317 Mouse
- Unigene: 428027 Human
see all -
Form
There are 4 isoforms produced by alternative splicing. -
Alternative names
- Homeobox protein PBX3 antibody
- MGC134053 antibody
- OTTHUMP00000064213 antibody
see all
Images
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Western blot - Anti-Pbx3 antibody (ab129478)All lanes : Anti-Pbx3 antibody (ab129478) at 1 µg (Milk blocking - 3%)
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
Lane 4 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe predicted molecular weight of Pbx3 is 47 kDa (SwissProt), however we expect to observe a banding pattern at 50 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab129478 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Pbx3 antibody (ab129478)All lanes : Anti-Pbx3 antibody (ab129478) at 1 µg (Milk blocking - 3%)
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
Lane 4 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe predicted molecular weight of Pbx3 is 47 kDa (SwissProt), however we expect to observe a banding pattern at 50 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
