Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [D2] to Hsp27
Suitable for: WB, IHC-P
Reacts with: Human, Pig
Isotype: IgG2b

Product name

Anti-Hsp27 antibody [D2]
See all Hsp27 primary antibodies
Description

Mouse monoclonal [D2] to Hsp27
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human, Pig
Immunogen

Recombinant fragment (His-tag) corresponding to Human Hsp27 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P04792

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Positive control
- IHC-P: Human breast cancer, glioma, brain, stomach and kidney tissues. WB: Human lung lysate; Pig muscle and heart lysates; Recombinant human Hsp27 protein.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

D2
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

Paraffin-embedded human breast cancer tissue stained for Hsp27 using ab239499 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Hsp27 antibody [D2] (ab239499)

Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/ml + Pig skeletal muscle lysate

Predicted band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

Paraffin-embedded human glioma tissue stained for Hsp27 using ab239499 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

Paraffin-embedded human brain tissue stained for Hsp27 using ab239499 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

Paraffin-embedded human stomach tissue stained for Hsp27 using ab239499 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Hsp27 antibody [D2] (ab239499)

Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/ml + Recombinant human Hsp27 protein

Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 23 kDa
Western blot - Anti-Hsp27 antibody [D2] (ab239499)

All lanes : Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/ml

Lane 1 : Human lung lysate
Lane 2 : Pig muscle lysate
Lane 3 : Pig heart lysate

Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

Paraffin-embedded human kidney tissue stained for Hsp27 using ab239499 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Hsp27 antibody [D2] (ab239499)

Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/ml + Pig heart lysate

Predicted band size: 23 kDa

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