Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TID1 antibody [EPR12414] (ab181024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12414] to TID1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TID1 antibody [EPR12414]
    See all TID1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12414] to TID1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • JAR, Jurkat, HeLa and K562 cell lysate. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-TID1 antibody [EPR12414] (ab181024)
    Western blot - Anti-TID1 antibody [EPR12414] (ab181024)
    All lanes : Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] (ab181024)
    Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] (ab181024)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

  • Immunoprecipitation - Anti-TID1 antibody [EPR12414] (ab181024)
    Immunoprecipitation - Anti-TID1 antibody [EPR12414] (ab181024)

    Lysate from Jurkat cells  (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Anti-TID1 antibody [EPR12414] (ab181024)
    Anti-TID1 antibody [EPR12414] (ab181024)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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