Anti-Hsp27 antibody [6H11] (ab190314)
Key features and details
- Mouse monoclonal [6H11] to Hsp27
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp27 antibody [6H11]
See all Hsp27 primary antibodies -
Description
Mouse monoclonal [6H11] to Hsp27 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Hsp27 aa 1 to the C-terminus. (Purified from E. coli).
Database link: P04792 -
Positive control
- WB: HeLa and HEK-293 cell lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6H11 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Hsp27 antibody [6H11] (ab190314) at 1/10000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 27 kDa
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Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp27 using ab190314 at 1/2000 dilution (green) and costained with chicken polyclonal antibody to Hsp60 at 1/2000 dilution (red). Counter-stained with DAPI.
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Immunofluorescent analysis of HeLa cells labeling Hsp27 with ab190314 at 1/1000 dilution (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue).