Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59) - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free
    See all NR1D1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18346-44] to NR1D1 (phospho S55 + S59) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T transfected with NR1D1 expression vector containing a GFP-tag®, whole cell lysate

  • General notes

    Ab251057 is the carrier-free version of ab191218. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251057 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)
    Western blot - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)
    All lanes : Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] (ab191218) at 1/5000 dilution (Purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a GFP-tag®, whole cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with NR1D1 expression vector containing a GFP-tag®, whole cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with NR1D1 expression vector containing a GFP-tag® whole cell lysate,then the membrane treated with Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab191218, the same antibody clone in a different buffer formulation.

     

  • ELISA - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)
    ELISA - Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)

    This data was developed using ab191218, the same antibody clone in a different buffer formulation.

    Direct ELISA analysis of ab191218 (0-1000 ng/ml) using the following antigens: NR1D1 phospho S55 + phospho S59 peptide, NR1D1 phospho S55 peptide, NR1D1 phospho S59 peptide and NR1D1 unmodified peptide at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 was used as secondary antibody.

  • Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)
    Anti-NR1D1 (phospho S55 + S59) antibody [EPR18346-44] - BSA and Azide free (ab251057)

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