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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp90 beta antibody (ab236282)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Hsp90 beta antibody (ab236282)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsp90 beta
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Zebrafish
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsp90 beta antibody
    See all Hsp90 beta primary antibodies
  • Description

    Rabbit polyclonal to Hsp90 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human Hsp90 beta aa 150-400.
    Database link: P08238

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse and rat lung lysate; Zebrafish lysate; K562, HepG2 and A549 cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Images

  • Western blot - Anti-Hsp90 beta antibody (ab236282)
    Western blot - Anti-Hsp90 beta antibody (ab236282)
    All lanes : Anti-Hsp90 beta antibody (ab236282) at 1/500 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Rat lung lysate
    Lane 3 : Zebrafish lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab236282)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab236282)

    4% formaldehyde-fixed, 0.2% Triton X-100 permeabilised MCF7 (human breast adenocarcinoma cell line) cells labeling Hsp90 using ab236282 (green) at 1/66 dilution in ICC/IF. Secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counter stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab236282)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab236282)

    Paraffin-embedded human pancreatic cancer tissue stained for Hsp90 using ab236282 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected vy a biotinylated secondary antibody and visualized using an HRP conjugates SP system.

  • Western blot - Anti-Hsp90 beta antibody (ab236282)
    Western blot - Anti-Hsp90 beta antibody (ab236282)
    All lanes : Anti-Hsp90 beta antibody (ab236282) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 83 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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