Anti-Hsp90 beta antibody (ab236282)
Key features and details
- Rabbit polyclonal to Hsp90 beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Hsp90 beta antibody
See all Hsp90 beta primary antibodies -
Description
Rabbit polyclonal to Hsp90 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Rabbit, Horse, Chicken, Cow, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Hsp90 beta aa 150-400.
Database link: P08238 -
Positive control
- WB: Mouse and rat lung lysate; Zebrafish lysate; K562, HepG2 and A549 cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Hsp90 beta antibody (ab236282) at 1/500 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Zebrafish lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 83 kDa
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4% formaldehyde-fixed, 0.2% Triton X-100 permeabilised MCF7 (human breast adenocarcinoma cell line) cells labeling Hsp90 using ab236282 (green) at 1/66 dilution in ICC/IF. Secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L). Counter stained with DAPI (blue).
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Paraffin-embedded human pancreatic cancer tissue stained for Hsp90 using ab236282 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected vy a biotinylated secondary antibody and visualized using an HRP conjugates SP system.
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All lanes : Anti-Hsp90 beta antibody (ab236282) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa