Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit (ab277453)
Key features and details
- Sensitivity: 0.068 ng/ml
- Range: 0.069 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.068 ng/ml -
Range
0.069 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100.6 73% - 132% Plasma 87.53 70% - 119% Cell culture media 80.54 70% - 92% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit (ab277453) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Junctional Adhesion Molecule 2/JAM-B in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Junctional Adhesion Molecule 2/JAM-B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Junctional Adhesion Molecule 2/JAM-B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Junctional Adhesion Molecule 2/JAM-B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Junctional Adhesion Molecule 2/JAM-B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human Junctional Adhesion Molecule 2/JAM2 Antibody 2 vials Anti-Human JAMB coated Microplate 1 unit Human Junctional Adhesion Molecule 2/JAM-B standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May play a role in the processes of lymphocyte homing to secondary lymphoid organs. -
Tissue specificity
Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Alternative names
- C21orf43
- CD 322
- CD322
see all -
Database links
- Entrez Gene: 58494 Human
- Omim: 606870 Human
- SwissProt: P57087 Human
- Unigene: 517227 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.