Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-ADRM1/ARM-1 antibody [EPR11450(B)]
See all ADRM1/ARM-1 primary antibodies
Description

Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 350 to the C-terminus.
Positive control
- WB: HEK293T, K562, Raji, HeLa, Raw264.7 and NIH 3T3 cell lysates. IHC-P: Human testis tissue, Human thyroid gland carcinoma tissue. Flow Cyt: HeLa cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product was previously labelled as ADRM1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11450(B)
Isotype

IgG
Research areas

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ADRM1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Lanes 1-4: Merged signal (red and green). Green - ab157218 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

ab157218 Anti-ADRM1/ARM-1 antibody [EPR11450(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157218 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution

Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

Flow cytometric analysis of permeabilized Hela cells using ab157218 at 1/10 (red) or a rabbit IgG negative (green).
Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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