Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free
    See all ADRM1/ARM-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, K-562, HeLa and Raji cell lysates; Immunoprecipitation pellet from Raji cell lysate. Flow Cyt: K562 cells. IHC-P: human hepatocellular carcinoma tissue

  • General notes

    ab249293 is the carrier-free version of ab157185 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249293 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ADRM1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ADRM1 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This data was developed using ab157185, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab157185 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab157185 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    This data was developed using the same antibody clone in a different buffer formulation (ab157185).
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling ADRM1/ARM-1 with purified ab157185 at 1/50 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
    Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
  • Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    This data was developed using ab157185, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells labeling ADRM1/ARM-1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).
  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab157185, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab157185, the same antibody clone in a different buffer formulation.

     

  • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
    Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)

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