Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
Suitable for: WB, IHC-P, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-ADRM1/ARM-1 antibody [EPR11449(B)]
See all ADRM1/ARM-1 primary antibodies
Description

Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 100-200.
Positive control
- WB: HEK293T, K562, HeLa and Raji cell lysates; Immunoprecipitation pellet from Raji cell lysate. Flow Cyt: Permeabilized K562 cells. IHC-P: Human hepatocellular carcinoma
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as ADRM1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11449(B)
Isotype

IgG
Research areas

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ADRM1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Lanes 1-4: Merged signal (red and green). Green - ab157185 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

ab157185 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling ADRM1/ARM-1 with purified ab157185 at 1/50 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Flow cytometric analysis of permeabilized K562 cells labeling ADRM1/ARM-1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).
Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa
Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa
Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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