Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ADRM1/ARM-1 antibody [EPR11449(B)]
    See all ADRM1/ARM-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 100-200.

  • Positive control

    • WB: HEK293T, K562, HeLa and Raji cell lysates; Immunoprecipitation pellet from Raji cell lysate. Flow Cyt: Permeabilized K562 cells. IHC-P: Human hepatocellular carcinoma

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as ADRM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11449(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ADRM1 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab157185 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab157185 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling ADRM1/ARM-1 with purified ab157185 at 1/50 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
    Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
  • Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

    Flow cytometric analysis of permeabilized K562 cells labeling ADRM1/ARM-1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).

  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

  • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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