Anti-Nephrin antibody - C-terminal (ab183099)
Key features and details
- Rabbit polyclonal to Nephrin - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nephrin antibody - C-terminal
See all Nephrin primary antibodies -
Description
Rabbit polyclonal to Nephrin - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide within Human Nephrin aa 1200 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004637)
Database link: O60500 -
Positive control
- Mouse spleen and mouse kidney tissue lysates; Human liver cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nephrin antibody - C-terminal (ab183099)All lanes : Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Predicted band size: 134 kDa
Exposure time: 1 minute8% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody - C-terminal (ab183099)Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Nephrin with ab183099 at 1/50 dilution. Image on the right is treated with the synthetic peptide.
