Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
![Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-326723-anti-mmp9-antibody-ep1255y-immunohistochemistry.jpg "Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1255Y] to MMP9 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free
See all MMP9 primary antibodies -
Description
Rabbit monoclonal [EP1255Y] to MMP9 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U937 cell lysate. IHC-P: Human tonsil and lymphoma tissues.
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General notes
ab219372 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1255Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137867).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-2-MMP9Primaryantibodiesab1378671.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137867).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-291606-ab137867IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)This IHC data was generated using the same anti-MMP9 antibody clone, EP1255Y, in a different buffer formulation (cat# ab137867).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)](https://www.abcam.com/ps/products/219/ab219372/Images/ab219372-5-benefits-of-recombinant-antibodies.png)
Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (ab219372)
