Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21062] to MRP1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MRP1 antibody [EPR21062] - BSA and Azide free
    See all MRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21062] to MRP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung, gastric and esophagus cancer tissue. WB: HeLa and A549 cell lysates. ICC/IF: HeLa, A549 and BxPC-3 cell lysates.

  • General notes

    ab234098 is the carrier-free version of ab233383.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Western blot - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    All lanes : Anti-MRP1 antibody [EPR21062] (ab233383) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MRP1 knockout HeLa cell lysate
    Lane 3 : Wild-type A549 cell lysate
    Lane 4 : MRP1 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 172 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab233383).

    Lanes 1-4: Merged signal (red and green). Green - ab233383 observed at 250 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab233383 Anti-MRP1 antibody [EPR21062] was shown to specifically react with MRP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265256 (knockout cell lysate ab257242) was used. Wild-type and MRP1 knockout samples were subjected to SDS-PAGE. ab233383 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and adjacent non-cancerous lung tissue (A) labeling MRP1 with ab233383 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233383).

  • Immunocytochemistry - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunocytochemistry - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    This data was developed using the same antibody clone in a different buffer formulation (ab233383).

    ab233383 staining MRP1 in wild-type HeLa cells (top panel) and ABCC1 knockout HeLa cells (ab265256) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab233383 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer (B) and adjacent non-cancerous stomach tissue (A) labeling MRP1 with ab233383 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining in human gastric cancer tissue (B) with weak staining in its adjacent non-cancerous tissue (A)
    (PMID:23667609) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233383).

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    Immunofluorescent analysis of 100% methanol-fixed BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling MRP1 with ab233383 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in BxPC-3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233383).

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    Immunofluorescent analysis of 100% methanol-fixed A549 (human lung carcinoma cell line) cells labeling MRP1 with ab233383 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233383).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

    Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and adjacent non-cancerous esophagus tissue (A) labeling MRP1 with ab233383 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous and cytoplasmic staining in human esophageal cancer tissue (B) while staining is weak in its adjacent noncancerous tissue (A) (PMID: 26870278) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233383).

  • Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)
    Anti-MRP1 antibody [EPR21062] - BSA and Azide free (ab234098)

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